Journal: Nature Communications

Article Title: Microglia are an essential component of the neuroprotective scar that forms after spinal cord injury

doi: 10.1038/s41467-019-08446-0

Figure Lengend Snippet: The CSF1R inhibitor PLX5622, but not PLX73086, crosses the blood–spinal cord barrier to deplete virtually all microglia. a – c Representative confocal images of CD11b and P2ry12 immunostainings showing the almost complete elimination of microglia in the spinal cord of naïve (uninjured) C57BL/6 mice after treatment with the CSF1R inhibitor PLX5622 compared to those fed PLX73086 or the control diet. Mice were killed after 21 days of treatment. d – h Quantification of microglia in the spinal cord of uninjured C57BL/6 mice treated with PLX5622, PLX73086 or the control diet ( d ), as well as at the lesion epicenter in Cx3cr1 creER :: R26 - TdT mice killed at 1 ( e ), 7 ( f ), and 14 ( g ) days post-injury (dpi) ( n = 4–5 mice per group/time point). i Quantification of the proportional area of spinal cord tissue permeable to FITC-conjugated lectin injected intravenously prior to tissue fixation. j Quantification of Ly6G + neutrophils at the lesion epicenter at day 1 post-SCI in mice treated with either PLX5622, PLX73086, or the control chow ( n = 4–5 per group). k Quantification of the number of granulo-myelomonocytic cells at the lesion epicenter at 7 and 14 dpi in Cx3cr1 creER :: R26-TdT :: LysM -eGFP mice treated with either PLX5622, PLX73086, or the control diet ( n = 4–5 mice per group/time point). For all injured mice, treatment was initiated 3 weeks before SCI and continued until sacrifice. Data are expressed as mean ± SEM. ** p < 0.01, *** p < 0.001, **** p < 0.0001, PLX5622 or 1 dpi compared to the control group; and && p < 0.01, &&&& p < 0.0001, PLX5622 compared with the PLX73086 group. Statistical analysis was performed using a one-way ( d – g, i , j ) or two-way ( k ) ANOVA followed by a Bonferroni’s post hoc test. Scale bar: ( a – c , in c ) 200 µm

Article Snippet: For the quantification of the BSCB permeability, the proportional area of tissue stained with the FITC-LEA lectin within the entire coronal section at the lesion epicenter was measured using images taken at ×20 magnification with the Zeiss Slide Scanner Axio Scan.Z1.

Techniques: Injection

Journal:

Article Title: Identification of cell surface glycoprotein markers for glioblastoma-derived stem-like cells using a lectin microarray and LC-MS/MS approach

doi: 10.1021/pr100012p

Figure Lengend Snippet: Carbohydrate specificities of the 16 lectins used for lectin microarray.

Article Snippet: Phaseolus vulgaris leucoagglutinin , PHA-L , Galβ (1–4)GlcNAcβ (1–6)Man , EY Laboratories Inc..

Techniques: Microarray, Plasmid Preparation